Molecular Weight of Poliovirus Ribonucleic Acid

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Molecular weight of poliovirus ribonucleic acid.

Purified poliovirus single- and double-stranded ribonucleic acids (RNA) were examined by electron microscopy. The length of both molecules was found to be 2.37 mum. The uncorrected sedimentation coefficient for single-stranded RNA is 33S, as compared to 27S for the RNA of tobacco mosaic virus. It is calculated from these results that the molecular weight of the sodium form of poliovirus is 2.6 ...

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Infectivity of Ribonucleic Acid from Poliovirus in Human Cell Monolayers

Ribonucleic acid prepared by the method of Gierer and Schramm from concentrated and partially purified types I and II polioviruses has been demonstrated to be infectious for HeLa and human amnion cells in monolayers. In areas of cytopathogenic action resulting from invasion of cells by RNA, intact poliovirus, of the type from which the RNA had been prepared, is present. The infectivity of the R...

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Molecular integrity of plant ribosomal ribonucleic Acid.

Thermal denaturation of plant ribosomal RNA followed by gel fractionation shows that although a large percentage of molecules contain breaks in the polynucleotide chain, 25S and 18S RNAs do exist as unique molecular species. Values for the rate constant of hydrolysis under routine denaturing conditions are of the order of 10(-7) to 10(-8) sec(- 1) and these are shown not to be a result of ribon...

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Interspersion of repetitive and single-copy sequences in nuclear ribonucleic acid of high molecular weight.

Nuclear RNA of high molecular weight, approximately 15 to 30 x 10(3) nucleotides long, isolated from rat ascites cells, has been shown previously to contain both middle repetitive and single-copy sequences. However, it was not known whether these sequences were located on separate molecules or whether they were interspersed within the same molecule. The present communication distinguishes betwe...

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 1969

ISSN: 0022-538X,1098-5514

DOI: 10.1128/jvi.4.4.475-479.1969